PhanerochaeteV1, Main, Exploration, bibRecord, 000100

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.

Identifieur interne : 000100 ( Main/Exploration ); précédent : 000099; suivant : 000101

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.

Auteurs : Quoc-Thai Nguyen [Pays-Bas, Italie, Viêt Nam] ; Elvira Romero [Pays-Bas] ; Willem P. Dijkman [Pays-Bas] ; Suzan Pantaroto De Vasconcellos [Pays-Bas, Brésil] ; Claudia Binda [Italie] ; Andrea Mattevi [Italie] ; Marco W. Fraaije [Pays-Bas]

Source :

Biochemistry [ 1520-4995 ] ; 2018.

RBID : pubmed:30272958

Descripteurs français

KwdFr :
- Alcohol oxidoreductases (composition chimique), Alcohol oxidoreductases (génétique), Alcohol oxidoreductases (métabolisme), Catalyse (MeSH), Conformation des protéines (MeSH), Cristallographie aux rayons X (MeSH), Glycérol (métabolisme), Ingénierie des protéines (méthodes), Modèles moléculaires (MeSH), Mutagenèse dirigée (MeSH), Mutation (MeSH), Phanerochaete (enzymologie), Spécificité du substrat (MeSH), Structure moléculaire (MeSH).
MESH :
- composition chimique : Alcohol oxidoreductases.
- enzymologie : Phanerochaete.
- génétique : Alcohol oxidoreductases.
- métabolisme : Alcohol oxidoreductases, Glycérol.
- méthodes : Ingénierie des protéines.
- Catalyse, Conformation des protéines, Cristallographie aux rayons X, Modèles moléculaires, Mutagenèse dirigée, Mutation, Spécificité du substrat, Structure moléculaire.

English descriptors

KwdEn :
- Alcohol Oxidoreductases (chemistry), Alcohol Oxidoreductases (genetics), Alcohol Oxidoreductases (metabolism), Catalysis (MeSH), Crystallography, X-Ray (MeSH), Glycerol (metabolism), Models, Molecular (MeSH), Molecular Structure (MeSH), Mutagenesis, Site-Directed (MeSH), Mutation (MeSH), Phanerochaete (enzymology), Protein Conformation (MeSH), Protein Engineering (methods), Substrate Specificity (MeSH).
MESH :
- chemical , chemistry : Alcohol Oxidoreductases.
- chemical , genetics : Alcohol Oxidoreductases.
- chemical , metabolism : Alcohol Oxidoreductases, Glycerol.
- enzymology : Phanerochaete.
- methods : Protein Engineering.
- Catalysis, Crystallography, X-Ray, Models, Molecular, Molecular Structure, Mutagenesis, Site-Directed, Mutation, Protein Conformation, Substrate Specificity.

Abstract

Glycerol is a major byproduct of biodiesel production, and enzymes that oxidize this compound have been long sought after. The recently described alcohol oxidase from the white-rot basidiomycete Phanerochaete chrysosporium (PcAOX) was reported to feature very mild activity on glycerol. Here, we describe the comprehensive structural and biochemical characterization of this enzyme. PcAOX was expressed in Escherichia coli in high yields and displayed high thermostability. Steady-state kinetics revealed that PcAOX is highly active toward methanol, ethanol, and 1-propanol ( k_cat = 18, 19, and 11 s^-1, respectively), but showed very limited activity toward glycerol ( k_obs = 0.2 s^-1 at 2 M substrate). The crystal structure of the homo-octameric PcAOX was determined at a resolution of 2.6 Å. The catalytic center is a remarkable solvent-inaccessible cavity located at the re side of the flavin cofactor. Its small size explains the observed preference for methanol and ethanol as best substrates. These findings led us to design several cavity-enlarging mutants with significantly improved activity toward glycerol. Among them, the F101S variant had a high k_cat value of 3 s^-1, retaining a high degree of thermostability. The crystal structure of F101S PcAOX was solved, confirming the site of mutation and the larger substrate-binding pocket. Our data demonstrate that PcAOX is a very promising enzyme for glycerol biotransformation.

DOI: 10.1021/acs.biochem.8b00918
PubMed: 30272958
PubMed Central: PMC6210165

Affiliations:

Brésil, Italie, Pays-Bas, Viêt Nam

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000097
to stream Main, to step Curation: 000097

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.</title>
<author><name sortKey="Nguyen, Quoc Thai" sort="Nguyen, Quoc Thai" uniqKey="Nguyen Q" first="Quoc-Thai" last="Nguyen">Quoc-Thai Nguyen</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen </wicri:regionArea>
<wicri:noRegion>9747 AG Groningen </wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Scuola Universitaria Superiore IUSS Pavia , Piazza della Vittoria 15 , 27100 Pavia , Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Scuola Universitaria Superiore IUSS Pavia , Piazza della Vittoria 15 , 27100 Pavia </wicri:regionArea>
<wicri:noRegion>27100 Pavia </wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Faculty of Pharmacy , University of Medicine and Pharmacy at Ho Chi Minh City , 41 Dinh Tien Hoang Street, Ben Nghe Ward, District 1 , Ho Chi Minh City , Vietnam.</nlm:affiliation>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea>Faculty of Pharmacy , University of Medicine and Pharmacy at Ho Chi Minh City , 41 Dinh Tien Hoang Street, Ben Nghe Ward, District 1 , Ho Chi Minh City </wicri:regionArea>
<wicri:noRegion>Ho Chi Minh City </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Romero, Elvira" sort="Romero, Elvira" uniqKey="Romero E" first="Elvira" last="Romero">Elvira Romero</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen </wicri:regionArea>
<wicri:noRegion>9747 AG Groningen </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dijkman, Willem P" sort="Dijkman, Willem P" uniqKey="Dijkman W" first="Willem P" last="Dijkman">Willem P. Dijkman</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen </wicri:regionArea>
<wicri:noRegion>9747 AG Groningen </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="De Vasconcellos, Suzan Pantaroto" sort="De Vasconcellos, Suzan Pantaroto" uniqKey="De Vasconcellos S" first="Suzan Pantaroto" last="De Vasconcellos">Suzan Pantaroto De Vasconcellos</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen </wicri:regionArea>
<wicri:noRegion>9747 AG Groningen </wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biological Science , Federal University of São Paulo (UNIFESP) , Diadema , SP 09913-030 , Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Department of Biological Science , Federal University of São Paulo (UNIFESP) , Diadema , SP 09913-030 </wicri:regionArea>
<wicri:noRegion>SP 09913-030 </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Binda, Claudia" sort="Binda, Claudia" uniqKey="Binda C" first="Claudia" last="Binda">Claudia Binda</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biology and Biotechnology , University of Pavia , Via Ferrata 1 , 27100 Pavia , Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Biology and Biotechnology , University of Pavia , Via Ferrata 1 , 27100 Pavia </wicri:regionArea>
<wicri:noRegion>27100 Pavia </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mattevi, Andrea" sort="Mattevi, Andrea" uniqKey="Mattevi A" first="Andrea" last="Mattevi">Andrea Mattevi</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biology and Biotechnology , University of Pavia , Via Ferrata 1 , 27100 Pavia , Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Biology and Biotechnology , University of Pavia , Via Ferrata 1 , 27100 Pavia </wicri:regionArea>
<wicri:noRegion>27100 Pavia </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fraaije, Marco W" sort="Fraaije, Marco W" uniqKey="Fraaije M" first="Marco W" last="Fraaije">Marco W. Fraaije</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen </wicri:regionArea>
<wicri:noRegion>9747 AG Groningen </wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2018">2018</date>
<idno type="RBID">pubmed:30272958</idno>
<idno type="pmid">30272958</idno>
<idno type="doi">10.1021/acs.biochem.8b00918</idno>
<idno type="pmc">PMC6210165</idno>
<idno type="wicri:Area/Main/Corpus">000097</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000097</idno>
<idno type="wicri:Area/Main/Curation">000097</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000097</idno>
<idno type="wicri:Area/Main/Exploration">000097</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.</title>
<author><name sortKey="Nguyen, Quoc Thai" sort="Nguyen, Quoc Thai" uniqKey="Nguyen Q" first="Quoc-Thai" last="Nguyen">Quoc-Thai Nguyen</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen </wicri:regionArea>
<wicri:noRegion>9747 AG Groningen </wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Scuola Universitaria Superiore IUSS Pavia , Piazza della Vittoria 15 , 27100 Pavia , Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Scuola Universitaria Superiore IUSS Pavia , Piazza della Vittoria 15 , 27100 Pavia </wicri:regionArea>
<wicri:noRegion>27100 Pavia </wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Faculty of Pharmacy , University of Medicine and Pharmacy at Ho Chi Minh City , 41 Dinh Tien Hoang Street, Ben Nghe Ward, District 1 , Ho Chi Minh City , Vietnam.</nlm:affiliation>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea>Faculty of Pharmacy , University of Medicine and Pharmacy at Ho Chi Minh City , 41 Dinh Tien Hoang Street, Ben Nghe Ward, District 1 , Ho Chi Minh City </wicri:regionArea>
<wicri:noRegion>Ho Chi Minh City </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Romero, Elvira" sort="Romero, Elvira" uniqKey="Romero E" first="Elvira" last="Romero">Elvira Romero</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen </wicri:regionArea>
<wicri:noRegion>9747 AG Groningen </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dijkman, Willem P" sort="Dijkman, Willem P" uniqKey="Dijkman W" first="Willem P" last="Dijkman">Willem P. Dijkman</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen </wicri:regionArea>
<wicri:noRegion>9747 AG Groningen </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="De Vasconcellos, Suzan Pantaroto" sort="De Vasconcellos, Suzan Pantaroto" uniqKey="De Vasconcellos S" first="Suzan Pantaroto" last="De Vasconcellos">Suzan Pantaroto De Vasconcellos</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen </wicri:regionArea>
<wicri:noRegion>9747 AG Groningen </wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biological Science , Federal University of São Paulo (UNIFESP) , Diadema , SP 09913-030 , Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Department of Biological Science , Federal University of São Paulo (UNIFESP) , Diadema , SP 09913-030 </wicri:regionArea>
<wicri:noRegion>SP 09913-030 </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Binda, Claudia" sort="Binda, Claudia" uniqKey="Binda C" first="Claudia" last="Binda">Claudia Binda</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biology and Biotechnology , University of Pavia , Via Ferrata 1 , 27100 Pavia , Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Biology and Biotechnology , University of Pavia , Via Ferrata 1 , 27100 Pavia </wicri:regionArea>
<wicri:noRegion>27100 Pavia </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mattevi, Andrea" sort="Mattevi, Andrea" uniqKey="Mattevi A" first="Andrea" last="Mattevi">Andrea Mattevi</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biology and Biotechnology , University of Pavia , Via Ferrata 1 , 27100 Pavia , Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Biology and Biotechnology , University of Pavia , Via Ferrata 1 , 27100 Pavia </wicri:regionArea>
<wicri:noRegion>27100 Pavia </wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fraaije, Marco W" sort="Fraaije, Marco W" uniqKey="Fraaije M" first="Marco W" last="Fraaije">Marco W. Fraaije</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen </wicri:regionArea>
<wicri:noRegion>9747 AG Groningen </wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Biochemistry</title>
<idno type="eISSN">1520-4995</idno>
<imprint><date when="2018" type="published">2018</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alcohol Oxidoreductases (chemistry)</term>
<term>Alcohol Oxidoreductases (genetics)</term>
<term>Alcohol Oxidoreductases (metabolism)</term>
<term>Catalysis (MeSH)</term>
<term>Crystallography, X-Ray (MeSH)</term>
<term>Glycerol (metabolism)</term>
<term>Models, Molecular (MeSH)</term>
<term>Molecular Structure (MeSH)</term>
<term>Mutagenesis, Site-Directed (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Phanerochaete (enzymology)</term>
<term>Protein Conformation (MeSH)</term>
<term>Protein Engineering (methods)</term>
<term>Substrate Specificity (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alcohol oxidoreductases (composition chimique)</term>
<term>Alcohol oxidoreductases (génétique)</term>
<term>Alcohol oxidoreductases (métabolisme)</term>
<term>Catalyse (MeSH)</term>
<term>Conformation des protéines (MeSH)</term>
<term>Cristallographie aux rayons X (MeSH)</term>
<term>Glycérol (métabolisme)</term>
<term>Ingénierie des protéines (méthodes)</term>
<term>Modèles moléculaires (MeSH)</term>
<term>Mutagenèse dirigée (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Phanerochaete (enzymologie)</term>
<term>Spécificité du substrat (MeSH)</term>
<term>Structure moléculaire (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alcohol Oxidoreductases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Alcohol Oxidoreductases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alcohol Oxidoreductases</term>
<term>Glycerol</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Alcohol oxidoreductases</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Alcohol oxidoreductases</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Protein Engineering</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Alcohol oxidoreductases</term>
<term>Glycérol</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Ingénierie des protéines</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Catalysis</term>
<term>Crystallography, X-Ray</term>
<term>Models, Molecular</term>
<term>Molecular Structure</term>
<term>Mutagenesis, Site-Directed</term>
<term>Mutation</term>
<term>Protein Conformation</term>
<term>Substrate Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Catalyse</term>
<term>Conformation des protéines</term>
<term>Cristallographie aux rayons X</term>
<term>Modèles moléculaires</term>
<term>Mutagenèse dirigée</term>
<term>Mutation</term>
<term>Spécificité du substrat</term>
<term>Structure moléculaire</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Glycerol is a major byproduct of biodiesel production, and enzymes that oxidize this compound have been long sought after. The recently described alcohol oxidase from the white-rot basidiomycete Phanerochaete chrysosporium (PcAOX) was reported to feature very mild activity on glycerol. Here, we describe the comprehensive structural and biochemical characterization of this enzyme. PcAOX was expressed in Escherichia coli in high yields and displayed high thermostability. Steady-state kinetics revealed that PcAOX is highly active toward methanol, ethanol, and 1-propanol ( k<sub>cat</sub>
 = 18, 19, and 11 s<sup>-1</sup>
, respectively), but showed very limited activity toward glycerol ( k<sub>obs</sub>
 = 0.2 s<sup>-1</sup>
 at 2 M substrate). The crystal structure of the homo-octameric PcAOX was determined at a resolution of 2.6 Å. The catalytic center is a remarkable solvent-inaccessible cavity located at the re side of the flavin cofactor. Its small size explains the observed preference for methanol and ethanol as best substrates. These findings led us to design several cavity-enlarging mutants with significantly improved activity toward glycerol. Among them, the F101S variant had a high k<sub>cat</sub>
 value of 3 s<sup>-1</sup>
, retaining a high degree of thermostability. The crystal structure of F101S PcAOX was solved, confirming the site of mutation and the larger substrate-binding pocket. Our data demonstrate that PcAOX is a very promising enzyme for glycerol biotransformation.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30272958</PMID>
<DateCompleted><Year>2019</Year>
<Month>07</Month>
<Day>12</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>28</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-4995</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>57</Volume>
<Issue>43</Issue>
<PubDate><Year>2018</Year>
<Month>10</Month>
<Day>30</Day>
</PubDate>
</JournalIssue>
<Title>Biochemistry</Title>
<ISOAbbreviation>Biochemistry</ISOAbbreviation>
</Journal>
<ArticleTitle>Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.</ArticleTitle>
<Pagination><MedlinePgn>6209-6218</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.biochem.8b00918</ELocationID>
<Abstract><AbstractText>Glycerol is a major byproduct of biodiesel production, and enzymes that oxidize this compound have been long sought after. The recently described alcohol oxidase from the white-rot basidiomycete Phanerochaete chrysosporium (PcAOX) was reported to feature very mild activity on glycerol. Here, we describe the comprehensive structural and biochemical characterization of this enzyme. PcAOX was expressed in Escherichia coli in high yields and displayed high thermostability. Steady-state kinetics revealed that PcAOX is highly active toward methanol, ethanol, and 1-propanol ( k<sub>cat</sub>
 = 18, 19, and 11 s<sup>-1</sup>
, respectively), but showed very limited activity toward glycerol ( k<sub>obs</sub>
 = 0.2 s<sup>-1</sup>
 at 2 M substrate). The crystal structure of the homo-octameric PcAOX was determined at a resolution of 2.6 Å. The catalytic center is a remarkable solvent-inaccessible cavity located at the re side of the flavin cofactor. Its small size explains the observed preference for methanol and ethanol as best substrates. These findings led us to design several cavity-enlarging mutants with significantly improved activity toward glycerol. Among them, the F101S variant had a high k<sub>cat</sub>
 value of 3 s<sup>-1</sup>
, retaining a high degree of thermostability. The crystal structure of F101S PcAOX was solved, confirming the site of mutation and the larger substrate-binding pocket. Our data demonstrate that PcAOX is a very promising enzyme for glycerol biotransformation.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nguyen</LastName>
<ForeName>Quoc-Thai</ForeName>
<Initials>QT</Initials>
<AffiliationInfo><Affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Scuola Universitaria Superiore IUSS Pavia , Piazza della Vittoria 15 , 27100 Pavia , Italy.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Faculty of Pharmacy , University of Medicine and Pharmacy at Ho Chi Minh City , 41 Dinh Tien Hoang Street, Ben Nghe Ward, District 1 , Ho Chi Minh City , Vietnam.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Romero</LastName>
<ForeName>Elvira</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dijkman</LastName>
<ForeName>Willem P</ForeName>
<Initials>WP</Initials>
<AffiliationInfo><Affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>de Vasconcellos</LastName>
<ForeName>Suzan Pantaroto</ForeName>
<Initials>SP</Initials>
<AffiliationInfo><Affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Biological Science , Federal University of São Paulo (UNIFESP) , Diadema , SP 09913-030 , Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Binda</LastName>
<ForeName>Claudia</ForeName>
<Initials>C</Initials>
<Identifier Source="ORCID">0000-0003-2038-9845</Identifier>
<AffiliationInfo><Affiliation>Department of Biology and Biotechnology , University of Pavia , Via Ferrata 1 , 27100 Pavia , Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mattevi</LastName>
<ForeName>Andrea</ForeName>
<Initials>A</Initials>
<Identifier Source="ORCID">0000-0002-9523-7128</Identifier>
<AffiliationInfo><Affiliation>Department of Biology and Biotechnology , University of Pavia , Via Ferrata 1 , 27100 Pavia , Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fraaije</LastName>
<ForeName>Marco W</ForeName>
<Initials>MW</Initials>
<Identifier Source="ORCID">0000-0001-6346-5014</Identifier>
<AffiliationInfo><Affiliation>Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology Institute , University of Groningen , Nijenborgh 4 , 9747 AG Groningen , The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>10</Month>
<Day>16</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Biochemistry</MedlineTA>
<NlmUniqueID>0370623</NlmUniqueID>
<ISSNLinking>0006-2960</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>EC 1.1.-</RegistryNumber>
<NameOfSubstance UI="D000429">Alcohol Oxidoreductases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.1.3.13</RegistryNumber>
<NameOfSubstance UI="C019643">alcohol oxidase</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>PDC6A3C0OX</RegistryNumber>
<NameOfSubstance UI="D005990">Glycerol</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000429" MajorTopicYN="N">Alcohol Oxidoreductases</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002384" MajorTopicYN="N">Catalysis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005990" MajorTopicYN="N">Glycerol</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016297" MajorTopicYN="N">Mutagenesis, Site-Directed</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015202" MajorTopicYN="N">Protein Engineering</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2018</Year>
<Month>10</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2019</Year>
<Month>7</Month>
<Day>13</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2018</Year>
<Month>10</Month>
<Day>2</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">30272958</ArticleId>
<ArticleId IdType="doi">10.1021/acs.biochem.8b00918</ArticleId>
<ArticleId IdType="pmc">PMC6210165</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>FEBS J. 2009 May;276(10):2833-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19459938</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21460441</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Protein Sci. 1994 Dec;3(12):2245-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7756982</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Enzyme Microb Technol. 2014 Jul-Aug;61-62:7-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24910330</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Biochem. 1979 Nov;101(2):563-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">118005</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Microbiol. 1991 Oct;137(10):2381-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1770353</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Comput Chem. 2004 Oct;25(13):1605-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15264254</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Struct Biol. 2015 Oct;34:60-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26209821</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15299926</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS J. 2009 Dec;276(23):6857-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19843167</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Microbiol Biotechnol. 2013 Jun;97(12):5177-88</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23640366</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chem Soc Rev. 2008 Mar;37(3):527-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18224262</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1980 Dec;77(12):7099-101</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6261238</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2016 Jul 26;11(7):e0159476</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27458710</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Yeast. 1991 Apr;7(3):195-209</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1882546</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Angew Chem Int Ed Engl. 2007;46(24):4434-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17471485</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 1997 Dec 15;139(6):1419-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9396748</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2003 Dec 30;42(51):15179-88</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14690428</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2007 Oct;73(19):6241-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17660304</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):133-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20124693</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 1997 Nov 7;240(1):150-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9367901</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Microbiol Biotechnol. 2008 Feb;78(1):1-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18084756</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2007 Sep 21;372(3):774-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17681537</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 1999;131:1-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10494538</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Soc Trans. 1980 Jun;8(3):246-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7399046</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 1981 Apr 20;126(2):152-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6263691</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chem Rev. 2011 Jul 13;111(7):4073-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21682345</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chembiochem. 2016 Jul 15;17(14):1359-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27123962</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Microbiol Biotechnol. 2015 Aug;99(16):6617-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26153139</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Agric Food Chem. 2004 Jun 16;52(12):3749-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15186092</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15572765</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Microbiol Biotechnol. 2012 Dec;96(5):1243-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22290646</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Biochem Sci. 2000 Mar;25(3):126-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10694883</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 1996 Aug 27;35(34):11085-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8780511</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20124692</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2016 Feb 23;11(2):e0149846</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26905908</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Biochem. 1976 May 1;64(2):341-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6273</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Adv. 2009 Jul-Aug;27(4):489-501</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19374943</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Struct Funct Genomics. 2004;5(1-2):75-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15263846</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomol Concepts. 2014 Aug;5(4):299-318</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25372761</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):237-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24363322</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19461840</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 1994 Mar 1;50(Pt 2):178-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15299456</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Structure. 1997 Jul 15;5(7):907-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9261083</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Brésil</li>
<li>Italie</li>
<li>Pays-Bas</li>
<li>Viêt Nam</li>
</country>
</list>
<tree><country name="Pays-Bas"><noRegion><name sortKey="Nguyen, Quoc Thai" sort="Nguyen, Quoc Thai" uniqKey="Nguyen Q" first="Quoc-Thai" last="Nguyen">Quoc-Thai Nguyen</name>
</noRegion>
<name sortKey="De Vasconcellos, Suzan Pantaroto" sort="De Vasconcellos, Suzan Pantaroto" uniqKey="De Vasconcellos S" first="Suzan Pantaroto" last="De Vasconcellos">Suzan Pantaroto De Vasconcellos</name>
<name sortKey="Dijkman, Willem P" sort="Dijkman, Willem P" uniqKey="Dijkman W" first="Willem P" last="Dijkman">Willem P. Dijkman</name>
<name sortKey="Fraaije, Marco W" sort="Fraaije, Marco W" uniqKey="Fraaije M" first="Marco W" last="Fraaije">Marco W. Fraaije</name>
<name sortKey="Romero, Elvira" sort="Romero, Elvira" uniqKey="Romero E" first="Elvira" last="Romero">Elvira Romero</name>
</country>
<country name="Italie"><noRegion><name sortKey="Nguyen, Quoc Thai" sort="Nguyen, Quoc Thai" uniqKey="Nguyen Q" first="Quoc-Thai" last="Nguyen">Quoc-Thai Nguyen</name>
</noRegion>
<name sortKey="Binda, Claudia" sort="Binda, Claudia" uniqKey="Binda C" first="Claudia" last="Binda">Claudia Binda</name>
<name sortKey="Mattevi, Andrea" sort="Mattevi, Andrea" uniqKey="Mattevi A" first="Andrea" last="Mattevi">Andrea Mattevi</name>
</country>
<country name="Viêt Nam"><noRegion><name sortKey="Nguyen, Quoc Thai" sort="Nguyen, Quoc Thai" uniqKey="Nguyen Q" first="Quoc-Thai" last="Nguyen">Quoc-Thai Nguyen</name>
</noRegion>
</country>
<country name="Brésil"><noRegion><name sortKey="De Vasconcellos, Suzan Pantaroto" sort="De Vasconcellos, Suzan Pantaroto" uniqKey="De Vasconcellos S" first="Suzan Pantaroto" last="De Vasconcellos">Suzan Pantaroto De Vasconcellos</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000100 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000100 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PhanerochaeteV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:30272958
   |texte=   Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:30272958" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PhanerochaeteV1

This area was generated with Dilib version V0.6.37.
Data generation: Fri Nov 13 18:33:39 2020. Site generation: Fri Nov 13 18:35:20 2020

	Serveur d'exploration sur le phanerochaete
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le phanerochaete

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki